TrueLife - Doma Nunzio - Founder of MagicMyco, The MycoCoil, & The Cultivar Cup

Episode Date: April 14, 2023

One on One Video Call W/George https://tidycal.com/georgepmonty/60-minute-meetingSupport the show:https://www.paypal.me/Truelifepodcast?locale.x=en_US🚨🚨Curious about the future of psych...edelics? Imagine if Alan Watts started a secret society with Ram Dass and Hunter S. Thompson… now open the door. Use Promocode TRUELIFE for Get 25% off monthly or 30% off the annual plan For the first yearhttps://www.district216.com/https://magicmyco.org/https://magicmyco.com/Use promo code: MMFAM @ checkout.MagicMycoFam - The Cultivar CupFounder Doma is a mycologist and facilitator of TidalWave Cubensis (among others)Founder of Magic Mycology Online and inventor of Lab Tool Sterilizer MycoCoil™.Doma has a background in Bio Science, Art & Technology. Doma has become a pioneer in Cubensis genetic splicing, sub strain fusions, and isolations; while breeding for potency and contamination resistance. Through the team's lab and microscopy work they are leading the way and setting standards in the community.Doma and his Magic Myco team now document the Isolation research work and analyze the data with the MM team. HPLC chromatography is now available for potency testing on all fungi & extracts, and CRSPR is within reach for gene editing. These tools greatly help in the research, development, and quality control of the work so that it can be taken seriously in studies, treatments, clinical trials and of course legalization efforts.Doma Provides exceptional lab work with kind courteous customer service and strives to provide the highest in Mycology standards. He aims to push the Mycology hobbyist and the legalization awarness movements forward into a new age of understanding. All Mycology work is done in a professional, clean room environment & every spore is blessed with the good intent of its creator.• Creator of TidalWave Cubensis• Creator & Founder of Magic Myco, MYCOCOIL, The Cultivar Cup• 1st Place Strain @ Psilocybin Cup• Bio Science Student• CompTIA certified• Photography degree One on One Video call W/George https://tidycal.com/georgepmonty/60-minute-meetingSupport the show:https://www.paypal.me/Truelifepodcast?locale.x=en_USCheck out our YouTube:https://youtube.com/playlist?list=PLPzfOaFtA1hF8UhnuvOQnTgKcIYPI9Ni9&si=Jgg9ATGwzhzdmjkg

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Starting point is 00:00:01 Darkness struck, a gut-punched theft, Sun ripped away, her health bereft. I roar at the void. This ain't just fate, a cosmic scam I spit my hate. The games rigged tight, shadows deal, blood on their hands, I'll never kneel. Yet in the rage, a crack ignites, occulted sparks cut through the nights. The scars my key, hermetic and stark. To see, to rise, I hunt in the dark, fumbling, fear. Fearist through ruins maze, lights my war cry, born from the blaze.
Starting point is 00:00:40 The poem is Angels with Rifles. The track, I Am Sorrow, I Am Lust by Codex Seraphini. Check out the entire song at the end of the cast. Does I say, let me go live. Okay, ladies and gentlemen, welcome back to the True Life podcast. We are here with Dominoons. You're the founder of Magic Michael, the Michael Coil, and the Cultivor Cup. I'm so stoked you're here today, my friend.
Starting point is 00:01:25 I want everyone to learn about what you got going on over there. I want to take us through the website. More than that, can you maybe take a moment to introduce yourself and let people know what you're up to? Yeah, what's up, George? Thanks for having me on the show. This is really great. I'm over here in New York City. I know you're over in Hawaii.
Starting point is 00:01:42 So the morning time over there. Over here, it's getting towards the evening. But just so the audience knows, there is just this quick disclaimer. I have no idea what George is going to ask me. Nothing here has been pre-planned. So if you have some kiddos in the room, maybe you want to get the kids out of the room. This conversation might go TVMA pretty quick.
Starting point is 00:02:00 You know, I really have no idea. So just, you know, for safety and precautions, maybe you want to get the kiddos out of the room and leave this conversation to the adults. At that, you know, feel free to ask me whatever you want. Nice. Well, you would begin to touch upon the conference that you're at New York, with your friend over there.
Starting point is 00:02:21 Maybe you could talk a little bit about that. Right, because I was talking about the kids. So because we were over at the Horizons, New York City conference in December, and there was so many amazing panels and things going on there that we agreed with and we loved all the updates and the community, all this stuff.
Starting point is 00:02:38 Towards the ends of the conference, they asked a question to the audience, like, do you think kids should be participating in this research? Do you think this medicine is okay to give to kids? And a lot of the people said, Yes, and I'm of the opinion that kids, they're a little too young, their minds are a little bit too malleable, flexible. They're absorbing everything in, and they're not really ready for some of these experiences. So many of us have come to this mushrooms, entheogens, psychedelics industry through the cannabis industry.
Starting point is 00:03:11 Many of us started in the cannabis industry, and with cannabis, there have been many great breakthroughs with cannabis for kids and all kinds of disorders, you name it. But when it comes to this, it's very mental and on the mind, and I just don't think they're ready. So that was just one thing that I didn't agree with at that conference. Yeah, so that's why I asked the kiddos, you know. Having said that, you know, some of us are parents, you know, we want to educate our kids, how to be safe. You know, my daughter, she goes mushroom foraging with me all the time. She knows they're all poison and to do very good research on them and you have to cook them and all that stuff and how to take care of them. So, like, you know, to be very educated before you even step in that territory of consuming wild mushrooms, especially.
Starting point is 00:03:58 So, yeah, so, you know, when it comes to the kids, education, awareness, being honest with them is really key. Yeah, that was really well said, and I appreciate getting out in front of anything that could come our way because, you know, it's a live conversation. And sometimes an interesting conversation tends to find itself in some deeper waters that may be interesting. to some people, but consider it to other people. And, you know, maybe we could just stay on the topic of that conference you went to just for a moment. That was one of the things you disagreed with. What were some of the things that you did agree with? Well, there's a lot of great breakthroughs in the MDMA research in clinical trials.
Starting point is 00:04:37 They're like in phase three. That looks like that one is going to be, you know, going through pretty soon. Also, there's been lots of breakthroughs with psilocybin and mushrooms. Yeah, we, I mentioned. mentioned Gordo Tech. He's a good friend of mine. He actually has a free Patreon that you could look up all his posts for free and he has some very informative posts. And one that he made just recently was about some of these topics for legalization of psychedelics. And he had a couple links on there, like a bill tracker link where you can track all the active bills that are going
Starting point is 00:05:12 on in every state. See what's going on in your area and maybe do something about it. Reach out to some of the Congress people, some of the assembly people, and get involved in the legislative conversations on how to legalize this, or how to get involved with the bill directly. Yeah, that's a great point. Yeah, I think that's important. So there was a lot of stuff like that, science breakthroughs, legal conversations, and stuff like that.
Starting point is 00:05:42 Me and Gordo had a good time just, you know, kicking it and watching all the panels. It was pretty good. Yeah, it's such an exciting time. I'm curious, too, as someone who has, you know, while probably still has a foot, or maybe you don't, I don't know, maybe who had a foot in the cannabis industry, maybe you still do, and now you're branching out into this particular one. Do you see, what are some similarities and some differences? Well, as far as the testing procedures go, as far as HPLC, for those who don't know what that is, that's chromatography. It's a liquid chromatography method that we use to measure potency, toxicity, and other things. things. You know, there's some standard gear out there and standard consumables and a lot of the same
Starting point is 00:06:24 consumables and gear that will work for cannabis will also work for the mushrooms. So if some people are already set up to do, because there are many cannabis labs out there, but there are not many mushroom labs right now, it would be very easy to switch over a percentage of your operations, 10 or 20 percent, just into research, getting your methods developed, and all your protocols up to date so that when it does come to your state that you could be ready. Because I do think that it's going to be big. You know, it's good. It's, they call it the psychedelic renaissance.
Starting point is 00:06:59 You know, it's kind of like this term I see get thrown around a lot. But it is. It really is. It's like people are more interested now than ever. It's like a revival. Yeah, it's true. And there is so much research being done, not only on the front of mental illness,
Starting point is 00:07:18 but mental wellness, exploration, you know, I even see athletes finding a way to use it to improve their performance. So there's like a biohacking aspect to it. And there's a learning aspect to it. People are taking it and solving problems if we go back to Francis Crick and some of these scientists that were able to just take some time for quiet contemplation at some point in their journey, whether it was on, you know, an theogen of some kind. They're able to have these breakthroughs that they normally wouldn't have breakthroughs in. who knows what is headed our way, but what I do know for sure is that we need people doing what you're doing to figure out, hey, what exactly is in this particular strain or we need people to investigate that environment. And with that intro, maybe you can give some people some insight into what it is that HPLC does for the people that come to you.
Starting point is 00:08:11 Like, why are people coming to you and wanting to get these tests? So I run an online-based mycology work group through the Patreon where you get you support us by by pledging at different tier levels and you get some different benefits we have some lower tiers just supporting we have some middle tiers where we also send out monthly projects like spores and stuff where we could all work on the same genetic and kind of like research it together and then we have some top tiers which includes the testing now the HPLC testing is great because it breaks down the material the analytes individually so you can see them all separated individually it's a really great reagent kit and some other ones I know being
Starting point is 00:08:55 developed on the market right now like the miraculous silo Q test kit that will give you a good ballpark idea and it's a good inexpensive home test to use to kind of get a ballpark idea what you're working with and to see if you would want to send them in for HPLC testing now why would you want to send them in for HPLC testing because because it's more accurate. Like I said, you could separate all the analytes and get independently, not only the two major alkaloids
Starting point is 00:09:22 psilocybin and its active derivative psilocin, but also the minor alkaloids, which could lend possibly to some kind of entourage effect in the presence of the major alcoholids or an MAOI. So similar to cannabis, like with all the terapines and all the cannabinoids, each strain could have a particular kind of effect, you know, a good effect or a bad effect to some people.
Starting point is 00:09:48 So that's why the testing is so important so that people know what they're working with, because nobody wants to work with garbage strains, right? Us mycologist were making isolation. Some of them might look great on agar, and then we get to grain, and they don't even fruit right or something. So, like, you want to be periodically batch testing your strain, see what your gut, see if you want to continue with it. And, you know, beyond that, it's all about knowing.
Starting point is 00:10:14 how to dose. It's all about knowing how many milligrams of active compounds are in your one gram of mushrooms. One gram of mushrooms over here and one gram of mushrooms over there can be totally different. One could have one milligram of active alcoholids. The other one could have 30 or 40 milligrams of active alkaloids. So how can you tell somebody just here, go take this one without really knowing all that? Now sure, most of them are like averaged. You know, we could get into different topics with the genetics, multi-spor cultures versus isolate. You know, there'll be less variation with an isolate
Starting point is 00:10:52 than you'll get with a multi-spor culture. So usually we get our multispoors, we kind of go pheno hunting or crossing these strains to make like a pheno that we like. A phenotype is basically a strain of, that has a specific set of characteristics that we like, that we want to try to retain and keep. So these are the kind of projects. I do with the members online.
Starting point is 00:11:15 And, you know, I am not a trained chemist. I don't have a degree in chemistry or bioscience. I have a whole bunch of trade degrees, different phases in my life. I was a computer technician, artist. I was into some sciences and stuff like that. So, like, this kind of, like, it's a culmination of my life kind of, like, brought me to this place. Kind of a rough road brought me here. It wasn't like I was doing great in the cannabis industry and I was, you know, I'm a trained chemist and I know everything that there is to know about chemistry.
Starting point is 00:11:52 Far from it, you know, I was in a bad accident. When I was in my 20s, I almost lost my life and followed by some pretty heavy opiate addiction for many years, which which ruined my life in many ways, a few different times. And it wasn't, I had my first, you want to ask me about my first mushroom experience? Absolutely. Well, I was 18. I was a college student, right? And my girlfriend was going to Fordham University upstate. So I was up there with her in the dorm room. I was 18 years old with my girlfriend, you know, and she had this bag of mushroom. So we made tea out of it.
Starting point is 00:12:32 And it was an incredibly peaceful calm. I just remember sitting on the steps of this old college. You know the old college buildings, how they're like really like old looking and stuff. And we're sitting there and it's nighttime. And we're just looking at the trees, just the breeze going through the trees. And I can't even explain to you how euphoric that feeling was. But when I came back from that experience, I couldn't really explain it. I was a young guy.
Starting point is 00:12:58 I was messing around with other drugs. I was doing a lot of ecstasy at the time, maybe for the wrong reasons. I wasn't educated to MDMA or its healing potentials. I was just going to a rave or something, you know. And I had some very, very bad experiences. So that and then, so that was after college figure, 18, 19 years old. That kind of turned me off to that. And I kind of turned to heavier drugs, cocaine, heroin, like opiates and stuff like that.
Starting point is 00:13:28 So when I got to the accident and I was 21. and I had already, I was already on the methadone program, which is an opiate addiction program that you have to go to every day. And it's very miserable. So I got to that motorcycle accident and I couldn't get off the pain pills because now I was, I mean, I broke my pelvis. I fractured my skull. I thought I died. And I had a lot of mental instabilities after that, like for many years, like not even really believing that I even came. back and wondering where the hell I was like really crazy shit.
Starting point is 00:14:07 Yeah. And whatever other drugs I did just exacerbated that. But through my, through my fair, I would like to say moderate, but it was pretty much heavy, use of cannabis, it kind of helped curb some of those opiate withdrawals and stuff I was having. I switched up the program. I got onto Suboxone, which was a much easier program that you only have. to go to once a month. I used the cannabis and it wasn't until I was in my late 30s until life kind of pulled the carpet out from under me and my wife left me and I had nothing and I was in this decrepit apartment all by myself growing pot and doing BHO extractions
Starting point is 00:14:50 in the basement making gold hash oil at a time when it wasn't even in the High Times magazine. You never even store it and me and my buddies we used to buy these um sight glasses that used to go on the front of the furnace and we get a cork and put it on the top and like blast it straight through and make gold we had gold we had brown we had green we had all the best hash but it was basement hash you know what i mean right so long story short my life was pretty much crumble into pieces and just something out of nowhere was like what about mushrooms you know what about psychedelics and i went online i started looking up oh i saw it in the high times magazine that's why I saw it that's why I brought up the high science because I saw it in the
Starting point is 00:15:33 back of the hide there was an ad in the back of the high so there was it for a grow kit and it was expensive and I bought two or three of them and I failed miserably I got red mold in it I was hiding the bags like in my ceiling and stuff so when people came over they didn't see it I also had cannabis growing in the basement with my fertilizers and crap all over the place where I was getting like lipstick molds and right fusarium there's purple shit that like I not a successful for the whole first year and a half. Until I kind of got my shit together, I found a few good mentors,
Starting point is 00:16:07 one through kind of like a pen pal relationship on a bulletin board online, and then another one through Willie Miko. Willie Miko and his trip team family chat. I joined up with his thing like in the beginning when he first started doing it, and I learned so much from him, and it made it possible for me to do the same things
Starting point is 00:16:25 that he was doing and make it seem simple, you know? For a guy like me, I can figure it out. So, you know, with just like some basic science and chemistry, do all those different projects. So I got into his thing for a couple years. And because I was into cannabis, I loved crossing strains and I loved messing around with genetics. I did it with seed propagation, pollinating with the seeds. I also did it with the grafting, the cuttings of the clones together.
Starting point is 00:16:55 And I used to be into it. I made my own strain one time. I had it going. It was really good. I called the Tropic Dunder. It was badass. Somebody else had the name. It went extinct.
Starting point is 00:17:05 I fucked it up. So, yeah. Yeah, so through the cannabis, I got into the genetics. So when I came over to mushrooms, the first question I was asking was, how do I make a hybrid? How do we do this? You know, I started buying all the books. So for anybody out there who's new getting into mycology, like maybe for the first time, there's a couple really good books out there.
Starting point is 00:17:26 The Paul Stamish Mushroom Cultivator book is like a Bible. has it, the blue book. Radical Mycology is an excellent book. There's like two textbooks that we use in our, in our Discord and our Patreon all the time. The mushroom cultivator and the radical mycology. So I picked up the long story short,
Starting point is 00:17:43 I pick up all Paul Stamock stuff because I find out he's the man. And I read that book, and right there on page 27, he takes four cultures, and they're not even monocultures. They're dichariotic cultures. We can talk a little bit about the life cycle if you like. When a spore starts out, it creates a primary
Starting point is 00:18:06 mycelium, which is monocaryotic. Monocus is just one. It's just half of the genetics. It's one side of the genetics. So it needs to come together with another one, with another mate. Those two will fuse and form clamp connections between the hyphae, and they actually like meld, mesh into one, and will exchange the nuclei between them. And that's how they exchange. the gamets and the alleles and that creates a new strain so technically when you're working with the multi-sport culture you're making hundreds or dozens or hundreds of new strings and then we go isolate take our our phenos or whatever but I wanted to just like experiment with all this and a lot of the groups I got
Starting point is 00:18:49 involved in were like no you can't do this a few of them I just got booted right off you know like I don't want to throw out any names because I like them all and I'm still on the mall with secret names that nobody knows about it. But I think my first name on the Shermerie was the Joker. And all everybody did was laugh at everything I said. Maybe that was a bad idea. But so I was like, screw it. I'm going to do this shit anyway.
Starting point is 00:19:16 I'll make my own group. So I went on Facebook. I made a group, Magic Michael Pham Facebook group. You could go join it. And that's where it all started. I hooked up with a few people on there. Got some great genetics from all over, some really great places in Canada and the United States. Got some sports from some great people too.
Starting point is 00:19:36 I don't need to throw out names. We just had good genetics. So we started working with them and started all these different projects, working with the Monos, the Dicarion, starting in about tetrapola mycelium and unifactoral and bifactual genetics and how mutations and how all that fits in there. And these are topics that we still all hotly debated on the Discord and was still digging into and learning about it. So it's an ongoing evolution of our group. So basically over the past few years of doing it myself, we've created some isolations, hybrids, whatever you want to call them.
Starting point is 00:20:14 Some of which have done very well. One of them won this first psilocybin cup. I feel like I should throw that out there. Hosted and curated by Hafei Labs, Oakland Hafei Labs, excellent group of people who also do testing and research, just like I do. You should go check out, dig into all their stuff as well.
Starting point is 00:20:33 They put on the psilocybin cup, which I believe now is called the Haifa Cup. And they have conferences and do testing and all kinds of stuff. So definitely check them out. So I learned from everybody. I learned from watching everybody around the time that I found Oakland High Fay was doing that contest. I was looking into these things myself, like testing equipment,
Starting point is 00:20:55 because in making my hybrids and doing my projects, half the people would say that I did it, and half the other people would say that I didn't do it. And I really, because I'm not a trained scientist, I really had no good skills or resources to documents or to do a good experiment. So built the business up from my basement, from my basement apartment,
Starting point is 00:21:20 grew into a rather large business, especially after the psilocybin cup and stuff like that. And, you know, ever since that, we had to move into a commercial lab space because we hit a whole bunch of roadblocks. We hit a whole bunch of roadblocks. Yeah, you can't do this stuff out of a residential address, guys. You've got to have a commercial address. And anybody could do this research, you know. there are a couple different levels and there's different certifications. There's a certification I'm pursuing right now in New York State because cannabis is legal, right?
Starting point is 00:21:56 So I'm trying to get my ISO-17-025 accredation, which would make me a certified research laboratory testing laboratory. And then I could also send in my application to be state cannabis testing lab. And with that, would give us a whole bunch of new opportunities and maybe some stability so we could stay in this beautiful lab. We just moved into it's got a big 2,500 square foot lab. We got two HPLCs. We do potency testing, toxicity analysis. We could do DNA sequencing now. We also do those silo-Q tests, and we use this really nifty absorbance meter.
Starting point is 00:22:38 So we don't have to just look at the cue card anymore, the evaluation chart. we can actually get an actual score from the spectrometer from the color meter this is one made by i.O. Rodeo. Excellent, rather inexpensive. It runs on Python. You can install all your own calibration tables in here and make your own reagent kits and stuff like that. So those are all the kinds of things that I do with the with the page with the Magic Micro fam team on the Patreon and the Discord it started on the Facebook. Then after the psilocybin cup, we grew to the IG. And that's it.
Starting point is 00:23:17 Here we are. Here we are rocking on. Trying to, we got the Cultivar Cup coming up in June. The Cult of Our Cup is our event, is our event that we're hoping to get a good turnout this year, guys. So anybody can join. You know, we have three different entry fee levels. You could join as a non-member payful price. You could join as a member if I have price.
Starting point is 00:23:37 Or if you're on one of those top testing tiers, you could get them for free. And we're going to have five categories. We're going to have a few cool sponsors giving away great prizes. And just this is all, you know, it's not so much about who wins. It's about getting people involved, the education and the awareness. Naturally, the people who win will get some good promo and attention and stuff like that. And that's great. That's great for the whole community.
Starting point is 00:24:04 Like you said, more people need to be doing this, stepping out there. You know, some people are scared right now because of the legalities, a lot of gray and red tape, right? A lot of red tape and gray areas right now. We're trying to cut through. But if you're, if you set up a professional business and you pursue all the required and, you know, good, um, legal routes, um, that all protects you legally for what you're doing, especially, especially if you're doing it the way I'm doing it. Yeah, there's a right way and a wrong way to do. It's like, it's like drugs. This is a right way to do them and a wrong way to do them.
Starting point is 00:24:39 Yeah, you know, which paradigm are you going to live in? You want to live in the old paradigm and be like, oh, I'm a drug dealer selling shit in my basement. That's not legal. Or do you want to be like, shit, I ain't doing nothing wrong. Let's legalize this shit, you know, because it shouldn't be control one. It should be lower. We got to get it all. We got to deschedule, first of all.
Starting point is 00:24:57 I'm, you know, I'm trying to get involved in the bills in New York just to be a part of it so that we could deprioritize criminalization and decriminalize. set it and set a path for new modalities and different methods for setting up like Oregon is doing. Oregon, I think, was the first state to put a plan into motion, and now they're trying to get set up. I know a few labs have been approved, and people are getting set up to do the therapy, to do the sessions, to do the clinical, to do the testing. So that's pretty big. What's going on in Oregon. All of us should be paying attention to that. And also what's going on in your own state, what bills are in your own state, and who's,
Starting point is 00:25:37 who is putting them up, which assembly people are putting them up. And you want to write those people, you want to email those people. Tell them who you are. Tell them you support this movement. And we need better modalities and procedures in process for testing, for clinical research, for studies and all that. And it'll get past. It's going to get past.
Starting point is 00:26:00 I'm confident that all the psychedelics will be legalized by 2025. you know i'm curious so you had mentioned organ and these different states that are beginning to pass up you had mentioned that there's different kinds of people that are kind of coming to you for these different things there's different events does it kind of seem like this community is growing together the same way hyphae grows together and like yes we're building something right it's like a hive mind it's almost like that we're on another level almost it's like the internet it's like we're our own internet right it's like when you take these medicines they connect these neurons and make these pathways in your brain
Starting point is 00:26:35 that maybe weren't active or closed for a long time. And that's what fires up, lights up those emotions, those feelings, those thoughts that you have. And sometimes they just need to surface out and you need to purge a thought. Maybe you're not gonna actually purge a material like vomit. But maybe you have to purge, maybe you have to cry. Maybe you have to have a good cry.
Starting point is 00:26:56 Maybe you have to laugh really freaking hard at something that maybe you would take them too seriously. Maybe you have some serious issues. And like some of my friends, like, I need to go in my room for a little while in the dark and just go sit there and work some shit out. You know, there's really no such thing as a bad trip. Like those experiences, people will chalk up to a bad trip. And that's where it's really important about our professionalism and the way that we conduct our trials in our research and our experiments. You know, we have to do it professionally and aim to be as professional as we can and not some dark basement, you know, mycologist.
Starting point is 00:27:31 Although that's great and that's where we all came from. You know, in the end, in the end, all that darkness is going to get overtaken by a little bit of light. It's going to get legalized and it's just going to open up. Yeah, it's fascinating. I'm curious, what are the five categories? If someone enters the cult of our cup, what are the five categories are going to be judged in? Great question. So we got Cubensis.
Starting point is 00:27:55 Okay. We got exotics, which is anything other than cubensis. Your pancyans, your bisporas, all that will be, your retaliances. Tampanese, all that will be considered under exotics. Then we have extracts. Okay, so if you make an extract from your mushrooms, it could be a dry, it could be a wet extract. Quartersteps, and the last one is cannabis.
Starting point is 00:28:23 Have you seen a lot of extracts? I haven't seen, like, this is kind of a newer thing that's emerging. I haven't seen a lot of extracts. What's the word on that? No, and we just started doing this testing in the paper. year and I've seen some really great stuff over the past five years but and I wish I had some of a year now to test you know because we're not getting enough samples no we're not and the preliminary data coming in is very skewed like what we should
Starting point is 00:28:52 like COG which is the crystal of the gods extraction that people do with the mushrooms that make these crystal you know it was once believed to maybe be pure psilocided To make matters worse, people like Alexander Shulgin, who's looked up to as a god, we all love him naturally, but he's thrown out a few cockamamie ideas, you know, and he's a brilliant thinker. He was a brilliant chemist, but he didn't even have a good computer, you know. He didn't have an HPLC. He didn't have none of this stuff to confirm, really. He just knew how all the molecules were designed and had to design them and put them together and based it all off those theories. But he threw out a couple of cockerman things, one of which saying that it would be 99% pure psilocybin, which just bolstered the whole belief in the community. There's some other things going around too, like with additives and substrates. You know, I believe in using natural additives in your substrates. You know, some people like to use manure or not. You know, that's all great. Whatever your base substrate is, that's great. But then usually sometimes people will use a little additive.
Starting point is 00:29:59 And especially for testing purposes, I recommend that you set your experiments up with the control. All good experiments have a control. So in other words, you have your one that you do all the time, like your CVG or your DHSM substrate, dehydrated horse manure, or your core vermin, gypsum, right? That's like, say you're tried and true. So you'll have that. But then side by side, maybe now we'll have that, but with an additive. Maybe this one has more calcium.
Starting point is 00:30:25 Maybe this one has more triptophan. We tried to do that. We tried to give it 5HTP. heat. What I like to recommend is to give it an experiment with natural additives, calcium, nitrogen, phosphorus, magnesium, all these natural elements and trace minerals and elements. What I don't recommend is using your good drugs like 5 AMEO DMT that's very expensive in adding into a substrate in hopes of making a new compound because the preliminary results show It's not happening.
Starting point is 00:31:02 I've read about some psilomythoxin and talked to some guys that were using, I think it was Bufo, and they were feeding it to the mushrooms, and then they got, I don't know what their process was, but they had gone through and found a way for it to create this, this new substance. Have you heard about anything like that? Yeah, and I've seen many reports from labs just like mine. I think we've all tested it and know that it's bullshit. it. But none of us really want to touch the topic because of the legalities. However, I would like to think that the people running the church have, you know, they're trying
Starting point is 00:31:38 to protect themselves and their customers and they're just, they have good intentions and they're just trying to heal people and they're trying to protect themselves legally. You know, I've tested it. It's, I found psilocybin and solacea right over. Yeah, it's interesting to think about the way that it works in, and, and, you know, I've tested it. it's an interesting theory to think about. I guess, you know, I want to show some people an actual report that you guys have. Is there, and I have them all right here, right here. Cool, yeah, great.
Starting point is 00:32:09 Maybe we can, maybe we could go through this one here. This is the, what is this one? This is the April 29 right here. So maybe we could, you could kind of walk us through this and people could get a real understanding of what's happening there. Sure. So our reports are kind of evolving. And I mentioned that I'm seeking accredation to be a certified ISO state lab, right? So in doing that, it has me upgrading some of my procedures and implementing some processes that are necessary.
Starting point is 00:32:44 And I'm very glad that I came across in this business, like new computer systems, a limb system, an ELN system. We have to have our own COAs. So these, which is a report, basically. Right. So these reports that you're showing right now is basically an evolution of the style of report that I have been making for the past year. Looks very nice. It's fancy. It has a nice little background overlay there with some mushrooms. We also test a fly agaric on that one. It has a flyagaric in the background. And the cannabis one, it has a cannabis leaf in the background. They look great. And they also pertain the information most of it that it needs to have. But in the future, we might have an actual different actual official COA that will be, you know,
Starting point is 00:33:31 accepted on the state certification. We're getting there. This is very great. In this report, we basically give you some basic information on top, like the strain and the species and some basic attributes that the cultivator might have used, like the lighting or the substrate. Then we have a little alkaloid profile, those pie charts, which basically just breaks down all all the compounds that were found in the material and kind of breaks it down into a pie chart of, you know, those percentages, those ratios.
Starting point is 00:34:01 Then a little bit underneath that is the big table chart. That big table chart basically tells you individually one by one all the different analytes and the level at which they were found both in milligrams per gram and also in percent. a couple of bold numbers over there on the right, and that's your totals. And the one with the little purple highlight is your PCBE. Now, you see, the main thing that we're studying with these alkaloids are the two main alkaloids, the psilocybin and the solos. Sure, the miners are very important, and all the other stuff we haven't found yet is very important and should be listed on there and put into it, added up total, as it is there. And as it's done, in
Starting point is 00:34:50 cannabis tests and on cannabis labels. But when it comes to psilocybin in the bioavailability of psilocybin and the way that it converts to psilocin in the body, the different levels of psilocybin and psilocybin will give a potentially different effect, right? Because the psilocin is the active metabolite of the psilocybin. So if you consume more psilocybin, it's going to hit you faster. It's going to be a faster onset. It might be slightly more intense, but it'll also fade away quicker, much quicker. Whereas the psilocybin needs to still break down in your body. So when you take it, it's going to go through your liver, through your organs, it's going to get digested and then broken down into the psilocin, which will then break down further. And so those effects
Starting point is 00:35:42 will have a longer onset, like up to 90 minutes before it hits you. But it will last a much longer duration almost like a time release it just keeps kicking you so the combination of those two you know will pretty much determine how you know the bioavailability as you take it into your body so because of this it is good to give the added up total as i mentioned before but we also have what's called the pcBE which is the psilocybin equivalent value so celosin is so celosin is much more potent than psilocybin in its raw form. So we do a little calculation to convert based on the molecular weight, psilocybin into xylosin and solosin into psilocybin.
Starting point is 00:36:33 And we give you those two figures there. So the CB, the PCB is your psilocybin equivalent. And then next to it on the left, in a little smaller print there, is the PCNE, which is the psilocin equivalent. I made the psilocybin equivalent, the main. highlighted figure because I believe that is most important. So that figure should be more accurate in terms of how it's going to hit you and how it's going to feel. You know, on a side note, as you talk about this, I just my brain kind of goes in this direction. What if you
Starting point is 00:37:12 were to get like a good extract is that similar to like four ACOD DMT or these analogs? Like pure extract would be four ACOD DMT or something like it, right? Well, it depends on your process. If you use a lot, like with a lemon, with the whipetect, if you use an acidic solvent, it's going to convert most of it into your psilocin. So it's not going to be shelf stable for very long. Those water extracts, those blue water extracts, might kick your ass on the first day, but after about seven days they're going to oxidize and they're going to lose everything.
Starting point is 00:37:48 So that's why psilocybin we say is king. the King alkaloid because it's the most shelf stable. So just to get real fish, real quick, just to finish with the report because you wanted me to walk you through it. There's a little QR code there. So what the QR code is, is that is an NFT link. So if you were to click or to scan that QR code, it'll take you to the Ethereum blockchain on OpenC, where it links to a page with that strain, April 29. And in the description, there'll be a link to this very report that you're looking at. So the report links to the NFT and the NFT links to the report. We set this up in the beginning because we saw some other professional labs doing it for time stamping purposes.
Starting point is 00:38:32 Also to have the NFT as a collectible, a few other reasons. There's other ways to also do this. You know, there's DOI numbers, which is a meta-tag kind of number that professional researchers usually apply for. they could supply DOI numbers in their reports and research papers. And also as we do some of the genetic sequences, we're getting back the ascension numbers. So we're also adding those numbers onto the report too. So you might see it evolve a little bit over the court, you know, in the future as it has been this whole time. But if you go down, so this is like basically your nice cover page.
Starting point is 00:39:12 This is basically your cover page, my current COA, I sign it at the bottom. And then it goes on a few more pages. This is the actual Chem Station report. Now this isn't required. This is not required to give. Not in any form or fashion. But I like to show my work in the whole as I've been evolving and learning myself and developing our methods. I've been liking to show the actual chem station report, which tells you all the settings of the method, how I ran it. It's hard for me to see what it tells you all my equipment. And as you scroll down, it'll give you the actual chromatograph on one of those pages,
Starting point is 00:39:53 tell you how much I used, sample volume, the dilution fact is everything. So then it gives you the actual chromatograph right there. And it'll list for you the peaks. So each one of those peaks is another analyte that we can measure. How can we know? How can we measure?
Starting point is 00:40:11 Well, it's called a reference standard. So we buy these reference standards from chemical companies that, basically, that basically is a known amount of, say, psilocybin. So they'll send us one milligram in one millimeter of acetyanitin of psilocybin. So we know exactly how much is in there. So when we compare that one against the sample,
Starting point is 00:40:34 that's how we can measure and set up our calibration tables and give you an accurate result. So that's those peaks there, each one, the separation that we were talking about. The solvent goes, and passes over this column. It's called a stationary phase column that's filled packed with a whole bunch of silica gel.
Starting point is 00:40:55 And basically there's some different interactions in there based on the size, based on the electric properties, based on the dipole properties. And different analytes will get hung up in the column more than others. So some will come out faster and some will come out slower. So for this method, I have a pretty long HPL, column it's 150 millimeters because it needs that time to kind of separate and get on I mean we
Starting point is 00:41:24 have other columns and there's different methods this method that we're looking at here is called reverse phase chromatography okay we're not inventing anything new here this is a tried and true standard method that has been along for many many decades it's called a reverse phase chromatography and there's a couple ways to run in that in those methods you can run in an isocratic format or you can run in a gradient format. And that basically just has to do with your solvents and if you just stay the same or if you change from one to the other over the course of the run. So this is a very standard procedure, reverse phase chromatography. However, this particular psilocybin mushrooms or any psilocybin in general, there's just, like we said,
Starting point is 00:42:10 there hasn't been enough research. People think reverse phase isn't very good for this because it's the polar analytes and because they don't have very good retention on the column. But the columns have gotten so much better, even though the machines are still low, the columns have gotten so much better. The way that we've mixed our mobile phases have gotten simpler and more efficient. And the sample prep process, which is the biggest variable in testing between labs, is how do their sample prep process? Most sample prep processes are very easy, like with cannabis or some extracts.
Starting point is 00:42:43 when it comes to some extra, but when it comes to like psilocybin mushrooms, all the medicine is stuck inside the tissues, inside the fibrous chitin, the tissues, so it takes more energy to break them out. That's why we do double concoctions. That's why we do an alcohol concoction and then a water concoction or vice versa. That's why we do this because it extracts different solubles and insoluables from the material. Whereas if you did that with cannabis, you would extract too much shit. You would get an RSO or something because you would just take everything. The fats, the lipids. That's why we blast straight through with the BHO because we just want the liquid to just go past it, cold, all the medicines on the outside of the plant. It's just
Starting point is 00:43:27 going to strip it right off. We even freeze it to make it brittle and all those tricombs will just break right off and come right out. When it comes to mushrooms, it needs to soak, it needs to absorb, it needs to break that cell wall so the medicine could be released. In the beginning, now that was taking me two days to do now I see other people's methods and procedures and they were doing it much different than me and their scores were coming out lower so I think that maybe some of the extraction processes weren't done or maybe you know it's just now it's better but yeah now we got it down to two hours so that's that's great that's great for mushrooms I could do high
Starting point is 00:44:04 throughput all day long yeah so that's great so that's our reports and you know they're coming along we'll probably have a little upgraded one once we get the official COA kind of variety going. But that's it. So we try to implement a bunch of different things, the NFT, the timestamping, the full report, make a little cover page for it. So you get a lot for your money if you wanted to get a test. And the prices, with the new developments and efficiencies, the prices are coming down.
Starting point is 00:44:36 So we can offer better prices. That's why I encourage people to get signed up so you can get a discount or you can get you know, some testing for free, get involved with our members, and make some friends, share some projects. You don't have to either. If you want to do a private and anonymous, that's fine. You know, we have a spot for you could get a private report
Starting point is 00:44:52 or under the reporting name on the forum. You could just put anonymous, you know, some people that might be what they want and that's okay. You know, all the data, it goes far in helping us. Because like we were saying, there's just, it's not that these processes aren't known or that they haven't even been done before. There's just not enough.
Starting point is 00:45:11 You know, how can you just do one stem and cap experiment and say that's it? Stems are stronger and cap or vice versa or whatever. You know, you've got to do it over and over and over on a lot of different strains. And then over time, we're going to compile hypothesis and see trends and be able to chart all these great things. So that's where we're at. We're basically in a spot of going professional, right? It's the way I like to think about it. Yeah.
Starting point is 00:45:38 I think the whole community or the whole space is in that. same spot. Like everybody, it's new, it's novel, and people are traversing the environment. Now look at this chart right here. You, you as someone who does this all the time, you could probably look at these profiles and be like, oh, that's a Cubenses or that's a Paniolos or... Right. Just look at it. Right. Yeah. And so this one is one of Cubantis right here? Yes, this is a Cubances stream. And then what is this like a is this a really good Cubances strain?
Starting point is 00:46:13 Is it these different bioscysteins and psilocybin? If you were going to create or maybe the strain that you created, did your bar chart look like this? Or like what are you looking for in a really good strain if you were just going to look at a profile? How would you know? Basically, I'm looking for a high psilocybin content. And everything else falls second for me.
Starting point is 00:46:34 We know the other minor alkaloids where they are. There are a few unknown ones and a few mystery ones, especially with some of the exotics. A couple in particular are like azorescence and italiances. We're also finding trace amounts of MAOIs. MAOIs are fun to research because they're so fluorescent and they fluoresce under a UV light. So if you do a methanol extraction of your mushroom material and then look at it under a UV light, sometimes you'll see it could change color. Contaminations could also fluoresce too, so these are some things that we're looking into now.
Starting point is 00:47:10 But the MEOIs are present in some varieties of mushrooms. It's such a low level that it might not really do much. But if you take everything and put it together, that entourage effect as a whole might add up to be something special and substantial. Yeah, we're in a big experimental phase right now. We're collecting data, developing these methods. You know, we have a very old, open source sharing approach to it.
Starting point is 00:47:37 You know, some people don't or maybe they're just not at that level yet. But I think that people should share, you know, I mean, we're a pretty big community and we want to be part of it. We want to be part of it as it becomes legalized and so they don't like ruin it. Like some, you know, we should learn from our experiences with the cannabis industry and all the trials and tribulations we went through there with legalization and testing and, you know, all the state by state stuff. I got to say really stuck. We got to just deschedule it and things will move faster. But it's great that we have all these bills going now. New York that I know of has three going right now,
Starting point is 00:48:14 one of which is looking really good. So, you know, who knows? It could be happening really soon. I want to be ready. Yeah, I had another question. Yeah, you will be, I think. You guys are at the forefront and there's some great people working in here. I'm curious.
Starting point is 00:48:29 I'm just putting on my curiosity hat. But it seems to me that when we look at cannabis and if we're using cannabis as somewhat of a model, you know, different strains tend to have different attributes or characteristics about them. And I'm wondering, maybe in the future we're going to be able to see, oh, well, this particular strain of mushrooms may help people with Alzheimer's, or this particular strain, maybe good people with epilepsy,
Starting point is 00:48:53 or maybe this particular strain is something that's been really helpful for people with PTSD. Is that something that could be working in the future or something you see on the horizon? Yes. Short answer, yes. Short answer, yes. To a point, to a point.
Starting point is 00:49:08 Because there is, it is very subjective and depends on the person's mental state. And many of these drugs can sometimes cause the same exact reactions in people. So, you know, more study is needed. A lot of times it's subjective depending on what's going on your life, what's on your mind. And although, yeah, like LSD is going to be much different than like, you know, psilocybin or, MDMA, sure, it's going to be different, but it might trigger like the same kind of response, emotional response or, you know, physiological response in your body. Having said that, yes, I do think that we will find out in the future some more details about
Starting point is 00:49:52 this one's good for this element or this one's good for that element. Maybe this one has more beta glucan, so it's good for this, or, you know, this one has more, you know, maybe we'll find out because we know more about the pest, pathway now. So Bayosystem and Norbaeosystem is actually part of the pathway. And the psilocybin and psilocybin can actually go back and forth, which is kind of interesting. And I also find it interesting that psilocybin as it degrades can turn into selosin, but it could also turn into norcelosin. And Bayocyssin can turn into psilocybin. So we might notice some trends, like if it had a really high Bayo system, like maybe we picked it mature or or vice versa. Like if it has a
Starting point is 00:50:34 really high psilocin content and the psilocybin is really low like my TW4 batch on this last report it was because I let it go too long I let it go to the point where they were turning black and they were basically rotting and senessing so when some people see that really black bluing they think I got the strongest shit maybe yeah maybe it was there but if you see that that means it's already degrading and losing what it had so some of the strongest highest testing strains that I have tested have come to me with almost no bruising because they were handled correctly. And and and and these pancyans are just flying off the charts. And if you ever seen a cubensis next to a pancyon, you know,
Starting point is 00:51:18 cubensis is fibrous. They could be very large and fibrous. A lot of material there, right? A lot of chitin. But with these pancyans, it's almost like tissue paper. There's like nothing there. Like you can't say to go do the same dehydrated technique because if you put those in for 24 hours, they might get dried to a crisp and take out all the alcoholids. Like, they dry really
Starting point is 00:51:38 fast. And because there's almost no material there, they just leave behind a lot of alcoholids. And they test really high. So I think that's part of the reason. But having said that, we were also talking about a couple other strains, like the Azis and a couple others, which were finding this other molecule that I don't know what it is yet. And I'm checking all the other ones that come after solosin. And I still don't know what it is. And is this big mysterious woodlo- paralysis thing that people are talking about a lot. So maybe that could be the peak. And I'm trying to figure out what that is. It's not the serotonin. It's not the TMT. It's not the DMT. I looked into all those. It's not. It's this very weird peak. Do you have access to all my reports right now?
Starting point is 00:52:21 Can you bring up an ASE report? I think so. Yeah. That's all right. All the reports are available. Yeah, I can do it right here. One of these ones right here? Yeah, we did some good ones this month. Dehydration experiment with a freeze dryer versus dehydrated. Now I'm getting on topic. The peanuts. Is there any Azis on there? No, we got Flyer Garrick, though. It was the month before.
Starting point is 00:52:51 So if you go up to the hamburger bar on the top and click on journals, reports, lab journal. And then go to February. I might have to just like, go back. Go back up. Yeah, right there. Labor Reports. Right here,
Starting point is 00:53:12 back on. Yeah, is there, like, icons in that window? How about reports? Yeah, right there, right there. There's March. Just scroll over to number two will be February. I don't see no, too. Oh, very good.
Starting point is 00:53:25 Yeah, there you go. Okay. Click, click, just click that. I'll go down. Do you see any links for Azis? February. February, we've got a lot of good stuff for the testers.
Starting point is 00:53:36 No, not that. Yeah. Two page results, 23rd in depth. Yeah, solve- Go all the way down. Sorry, guys. I want them to see this. Keep going, keep going.
Starting point is 00:53:47 Okay. Aziz right here. All right, try that one. Let's see if that one. I'm not sure if this one had it. Not all of the Azis samples had it, but some of the higher ones did. Go down to the chromatograph.
Starting point is 00:54:01 It might have been January. So you could reach all these reports. His computer's loading. Okay, so that one, see it has these other alkaloids. Yeah, it also, this one also had 5HT. Yeah. So that is another one, but it doesn't have the other peak that I was talking about. So right after the Salosin peak, the second to the last peak, on like, on a bunch of
Starting point is 00:54:28 azi samples, I get another peak right there, right? You might have to go to another month, like January. Okay, let's try it. The fan could do it if they want. All the reports are available on the website. There's links to all these reports and they could go check that out. So I give all that in our in-depth report. I'll probably always do that because I think that it's nice
Starting point is 00:54:49 for other testing labs to see the settings that I used. Yeah. But as far as certification, I might have a different, like official stamp approved like COA with the totals on it in the end that I actually give the customer. This one's great. Maybe I could still use this one. We'll find out.
Starting point is 00:55:08 But as we get better, we're adding the genetic information, DOI. We got the NFT on there. You know, maybe you win the cup and your strain is really good and you want the NFT just to have it, you know. Yeah. The NF beautiful idea. You know, the MAOI images that you talked about are probably a pretty awesome artwork, too.
Starting point is 00:55:28 Do you have those for NFTs? Like, you could almost sell those as artworks, you know, on top of it with that. I guess you're kind of doing that already. Yeah. Yeah, I'm not selling them. It's just like we're collecting them. So if you get the report, it's included. It's just included with your.
Starting point is 00:55:41 your report and you know wherever those links and the if we use it for the research what you know will get tagged along with it like we said before if you wanted an anonymous report and you don't want us to use it for our research that's fine too you know maybe you're doing batch testing and you don't want everybody to see everything you send it in because some you know is bunk and you just want to see what is bunk and what's not so you know just state that on your report and that's fine but we want to be able to share as much as possible we're asking people's to pledge as much as they can get on the top tier get the free entry to the cult of our cup,
Starting point is 00:56:15 and I'll make a nice written report, too, for it. It'll be sweet. You know, I wanted to touch base a little bit about the beginning of the conversation where I think a lot of us have gone through some times where we've found ourselves be pretty creative. Like, when you were making new strains and cannabis and stuff, and sometimes, like, you just come up with these ideas.
Starting point is 00:56:35 Like, I'm going to put these two together. I'm going to use this. The ideas I had that I tried in. It's all subjective for me, because I didn't have any control. group like that was I used like a really like an in 52 neodymium magnet and I exposed the cultivation to that and in my mind of course it turned out super awesome I'm wondering have you ever used like a high-powered magnet exposing those spores and stuff have you tried
Starting point is 00:56:58 you tried using those magnets before wonder if I could is there a way to turn my camera around um okay I don't think so I don't think there is but um I was going to turn my camera around so I could show you something in front of me but yes there's a big magnet right over here and I have magnets okay I've done electrical experiments I've done agar experiments with magnets agar all kinds of additives that you could possibly imagine I've put in copper wires in them and shock them yeah yeah you give it lightning so you know what I found with that one first of all it was a very dangerous device This is pre-mico coil, right?
Starting point is 00:57:42 So Michael Coil is my sterilizer device. It's nice, safe, patented technology. But before that, I was trying out this thing called a lightning plate. And we were shocking the Agar with stun guns, basically. And we were rebuilding them to make it this lightning plate. But it wasn't very safe. I don't know how I would have managed to get that to everybody. I think a few people did get one.
Starting point is 00:58:07 But yeah, they're pretty dangerous. We had a couple of electrodes on them. But what we found, and the Japanese have some good research on this with oyster mushrooms as well. And I think also the Bible has some good research on this too with the manna. So basically you get that microclimate in the negative ions in the air and you get that microclimate that like little fog and then you get some electricity and add a bolt of lightning in there. And when it hits the ground, you know what happens?
Starting point is 00:58:38 It fries the spot that it hits and it turns black and kills anything that was there. But then all around that area, like in a big ring, you could get all mushrooms literally like overnight, the mana, right? Wow. So, but here's the thing. Here's a trend that I'm noticing. Some of these faster, some of these faster fruiting strains, like the ones that pop up overnight, tend to come out way less potent, even some of sometimes inactive, whereas some of the strains that take longer to fruit, they like, they got to be in the tote for a while, they always come out stronger.
Starting point is 00:59:17 It's like they had more time to do their chemical factory process and make the good stuff, whereas with the lightning hitting it, yeah, we're doing Frankenstein, but we're just growing one up overnight and it doesn't have much time to make the good stuff. Yeah, it's fascinating. It might be good to feed like an army, but, you know, for, you know, for, you know, for, you If you want to have an experience, it would probably be pretty mild. But still, it was cool experiment. Yeah, we do all kinds of experiments like that on Agar.
Starting point is 00:59:48 Some of them, which I'd like to get back to. But I think the most important reason why you brought that up was because with the fusions and the hybrids. So there's a lot of rumors and not any good documented research online about people using snake venom to do this in the past. I never had any access to snake venom. I don't even know where to get that shit. So I have all antibiotics and gentomice and streptomyce and gentemise and I got a penicillin, a moxicillin. I got all these different antibiotics in my lab.
Starting point is 01:00:19 So I started experimenting with those. And some of them have gram negative and gram positive action. And you could look up what that means online, like it'll affect certain bacterial strains, but it won't with some molds or it will with both. You can look up with that means gram negative and positive. I found that by using a gram-negative and positive antibiotic like amoxicillin or penicillin that everybody has in their medicine cabinet, and using very, very little as an additive in my agar dish would basically make the strains grow slow.
Starting point is 01:00:59 Oh, I lost them. Power out. Okay. Can you still hear me? Yeah, there you go. So in the beginning when we were doing these sandwich. smashes and like we cut out two whole punches of a agar cut and put them together like a sandwich on top of the agar on top of this antibiotic agar so some of the some of the antibiotic additives
Starting point is 01:01:22 that only have like one way positive or negative action like the strain like very similar to activated charcoal like it might keep the contams away and you might get a nice rhizomorphic strain with these ropes but they'll be popping out here and there as they try to to get to the nutrients and there have a little bit of resistance against the antibiotic. But when you use the gram negative and positive one, like the penicillin or the amoxicillin, it makes everything grow slow and in place. Those rhizomorphs don't pop out so you don't have any chance for phenotypes here and there like all over the place. One very definite indicator is you could see a very clear distinct line that will form in between your
Starting point is 01:02:05 strains and that usually means that it didn't fuse, you know. But, but, you can see a very clear, distinct line that will form in between your strains, and that usually means that it didn't fuse, you know. But with this way, with the sandwich on the agar, with the antibiotic, I made it grow really slow, and it made it grow in place that it forced it to, like, grow over each other and meld. And then we grow that out. And then we take cuttings off that plate, like multiple cuttings and put that into an LC jar, swish roll up. And I let it go, like six months and colonizing 3D, like a 3D solution, like a 3D suspension. And that's how I made Trinity.
Starting point is 01:02:33 That's how I made Trinity. And it was, in the beginning, it was like so uniform and stable and looked really good. good and we've isolated it further since then, but it was almost nothing like that first one was really stable and pure. It was really good, so that's how we finished them. Since then, I've come up with some new techniques using the same kind of strategies, but with different buffers, use different solution. And I learned some of those biology packs from using other people's kits like the Odin, the Odin.com has some great, great, bacteria kits, mycelium kits, you name it, genetics kits, CRISPR kits.
Starting point is 01:03:14 And I started using all his kits and musseling around with the CRISPR genes and the GFP fluorescent proteins and some of these antibiotics. It could also get your DNA sequencing stuff there and everything. Yeah, so that's a good resource. So I just started playing with that and found that that worked. His transformation buffer. So he has a transformation buffer that you can use with say bacteria and a GFP protein to try to insert this plasmid DNA, which is basically one strand of the DNA in a circle. And what the buffer does is it softens.
Starting point is 01:03:52 Basically what I would imagine the snake venom would do would be to like soften that cell wall and disintegrate it to enough so that the two can come together or that the plasmid can be inserted into the nucleus. if it's two strains so that the two spores as to the two hype can come together and clamp and start sharing their nuclei and then and that's how you get your new strain and then i would do it in the buffer solution in test tubes and and do it that way so we just have a new way of doing it i still do the smash tech sometimes it's fun and fast and easy you know it's kind of like you know a little bit more very you could get some more varied you know some more variety it might be a little harder to isolate a really good one. That LC finishing trick is really good to do
Starting point is 01:04:37 that. Don't go right to the grain. Go to LC right up to the Agra. Man, it's exciting. It's like a, it's a whole new environment to be trying things and doing things. And you had mentioned if people get on a certain tier, they're able to follow along with some of your experiments so they could get a sport print
Starting point is 01:04:57 or something like that and then they could begin to do their own. Is that accurate? Every month? Yes. We have a small benefit, a medium benefit, and a large benefit. The small benefit is usually just like a 2-millimeter vial with some spores or L-C. The medium one is sometimes also like agar dishes, so you have like a full project. And then the highest ones, you get like two of everything, plus you could send in samples for testing. Perfect.
Starting point is 01:05:22 Perfect. I got to, Domai, I learned a lot by talking to you today. And it's, I'm really excited to get to see people at the forefront that are one of us, that are people that come up and maybe they didn't go to. Stanford and get a degree, but they're the people that are at the forefront that really care about creating things that make the world a little bit better. And I consider you one of those guys, man. I really appreciate it. I'm hopeful for the future. Maybe we can cover the Cultivore Cup or maybe we can talk more going forward to talk about new things. But before I let you go, man, what are you got coming up? Where can people find you? And what are you excited about?
Starting point is 01:05:56 Definitely. So we got the monthly testing. We got the Cultivar coming up. The deadline is June 1st. So if you have any questions about the Cultivar Cup, you can email me at Cultivar Cup at gmail.com. If you want to email me at my private email about anything whatsoever, it's DOMA, DOMA, at magicmiko.org. And you can go to magicmiko.org and find everything you need about testing there.
Starting point is 01:06:20 If you'd like to see my spore vendor shop, it's on magicmico.com. And magicmico.net is a page with all the links, is the link tree. You know what? I don't think we touched on the micro coil, man. People wanted to get into that or check it out. Can they buy that on your site?
Starting point is 01:06:37 Sure, they could buy it on the site. There's also a special site. They could buy it on the magic michael.com or they could also go to Michaelcoil. And if you're listening to this video, you're part of the fam now, so try to use coupon code M.M.FAMM. M.M. FAM. Okay, coupon code M.M.FAM.
Starting point is 01:06:57 Try that one. Nice. You heard it here, ladies and gentlemen. M.FAM. Use that code. Check out Domofoam. Follow him. Guys, he's got a lot of incredible things coming up. And I didn't even know about the Oakland team over there.
Starting point is 01:07:11 But I'm really thankful to get to talk to you. And I think everybody that's in this space should be having a spotlight on you, man, because we see good things coming out of the lab. And I'm stoked to be part of the M.M. fam. And looking forward to our future conversations, man. Thank you, George. I really appreciate you having me on. Yeah, man.
Starting point is 01:07:29 Pleasures all mine. That's all we got for today, ladies and gentlemen. Appreciate your time. All right. Let me end this one here. and then talk to you for one more.

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